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The elficacy of electrolyzed oxidizing water for inactivating Escherichia coli 0157:H7, Sabmmella enten'tfiis, and Listeria monoqytogenes was evaluated. A five-strain mixture of E. coli 0157:H7, S. entefia'dis, or L. mono- cytogenes of approximately 10“ CFU/ml was inoculated in 9 ml ot' electrolyzed oxidizing water (treatment) or 9 ml of sterile, deionized water (control) and incubated at 4 or 23°C for 0, 5, 10, and 15 mia; at 35°C for 0, 2, 4, and 6 min; or at 45°C for 0, 1, 3, and 5 min. The surviving population of eaeh pathogen at each sampling time was determined on tryptic soy agar. At 4 or 23°C, an exposure time of 5 min reduced the populations of all three pathogens in the treatment samples by approximately 7 log CFU/ml, with complete inactivation by 10 min of exposure. A reductîon of 27 log CPU/ml in the levels of the three pathogens occurred in the treatment samples incubated for 1 min at 45°C or for 2 min at 35°C. The bacterial counts of all three pathogens in contro] samples remained the same throughout the incubation at all four temperatures. Results indicate that electrolyzed oxidizing water may be a usel‘ul disinfectant, but appropriate applications need to be validated.

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Diego Fumaneri

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